MPs will question transport secretary Patrick McLoughlin about a recent government report on HS2.
He will face the Commons Transport Select Committee alongside the report's authors from KPMG on November 26th.
The study into HS2 originally concluded the project could provide up to £15 billion in annual benefits, but a Freedom of Information request by BBC Newsnight found previously undisclosed figures that showed potential losses in some areas.
Specifically, it uncovered regions unconnected to HS2 would lose money on an annual basis, with the most affected being around Aberdeenshire, which could miss out on up to £220 million.
Chair of the committee Louise Ellman said: "HS2 is a hugely significant and controversial investment. We are following up the questions we raised in our earlier report, in the light of significant new information."
Last week, it was claimed by Professor Henry Overman that important calculations by KPMG were "essentially made-up", which could stand to jeopardise the results concluded from such findings.
In the same week, the new chairman of HS2 Sir David Higgins was tasked with cutting down costs for the project - he has until March to report how it can be delivered within its budget.